Core technology(Process development for recombinant protein)
rProtein Manufacturing AD-Pure™ System
rProtein Manufacturing AD-Pure™ System
Most of all natural proteins in E. coli proteome, have pI vales below 7.0. ADfuse technology leads to pI 8.5~10.0 by reconstruction of fusion protein, therefore we can recover highly purified proteins not only from E. coli natural proteins but also from endotoxin, DNA, and lipids at first capturing step.
On-column Cleavage (ADzyme™)
We conduct on- column cleavage of fusion protein instead of elution at 1st capturing step. Our ADzyme™ exactly cut off the fusion partner from the interested protein.
We can usually obtain over 90% purity only at this 1st step of purification process.
Comparison for Technology
APTech’s proprietary process technology enables us to manufacture proteins 3~ 10 times cheaper than its peers.
- ITEM
- Host
- Final purification hGH per fermentation broth
- Purification Step
- APTech
- E.Coli
- 1.2g/L
- 2~3
- Company A
- E.Colii
- 0.3g/L
- 8~10
- Company B
- Yeast
- 0.15g/L
- 7~8
Contact research
OverviewOur Contract Research proceeds with three steps.
In Feasibility Study Step, we confirm whether or not our ADpure System™ is working for client’s proteins / peptides.
The following steps are Optimization of Process and Scale-up Studies.
At the completion of Contract Researh, client can choose license- in of the technology or contract manufacturing at APTech.
Procedure for CR
-
Pre Contract
- Consulting
- Signing a CDA & Proposal
-
Feasibility Study
- Contract
- Sample for Evaluation
- Study and Report (2~3 months)
-
Optimization
- Contract
- Sample for Evaluation
- Study and Report (2~3 months)
Scale-up
- Contract
- Sample for Evaluation
- Study and Report (2~3 months()
List of Our Protein Products
- Product name
- EGF
- VEGF
- FGF-1
- bFGF
- IGF-1
- PDGF-AA
- GM-CSF
- GLP1A
- hGH
- BMP2
- ProteinA
- ProteinG
- Full name
- Epidermal Growth Factor
- Vascular Endothelial Growth Factor
- Fibroblast Growth Factor-1
- Basic Fibroblast Growth Factor
- Insulin like Growth Factor-1
- Platelet Derived Growth Factor-AA
- Granulocyte Macrophage Colony Stimulating Factor
- Glucagon like Peptide 1-A
- human Growth Hormone
- Bone Morphogenetic Protein 2
- ProteinA
- ProteinG
- Supply Unit
- Contact us